The network includes four hospitals – Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre – West Park Healthcare Centre, the Toronto Rehabilitation Institute, and The Michener Institute, a post-secondary institution granting diplomas and certificates in health sciences and leadership.

In 1998, the Princess Margaret Cancer Centre joined, with the resulting institution named the University Health Network in 1999.

The Michener Institute for Education was originally established in 1958 and is the first non-medical unit to join the UHN.

[7] The program is led by infectious diseases specialist Andrew Morris,[8] who joined as founding director at its inception in 2009.

[9] During the COVID-19 pandemic, UHN received a $323,981 grant from the Public Health Agency of Canada's Immunization Partnership Fund to increase COVID-19 vaccine uptake among personal support workers and their patients.