The University established the Tuks Football Academy in 2002, originally playing in the SAFA Gauteng South Division.

In the 2003–04 season the University acquired Pretoria City FC's second division status, subsequently winning the Vodacom League play-offs and being promoted to the NFD in 2004–05.

In the 2008–09 season the club was a Nedbank Cup finalist losing to Premiership team Moroka Swallows 0–1.

[1] Following the 2011–12 season University of Pretoria gained promotion to the Premiership with Stephen Haupt as the head coach.

[4] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.