He played 16 seasons in the National Hockey League (NHL) for the Philadelphia Flyers, Ottawa Senators, Florida Panthers, Tampa Bay Lightning, Mighty Ducks of Anaheim, New York Rangers and Columbus Blue Jackets from.

At the beginning of the 2007–08 season, while with the Lightning, Prospal posted 12 goals and 10 assists making it his best career start.

On February 25, 2008, Prospal was dealt back to the Flyers for defenseman Alexandre Picard and a 2009 second-round draft pick.

[1] The Lightning signed Prospal to a four-year contract on June 30, in a deal worth $14 million overall to the player.

He also served as an assistant coach for the Czech national team in the World Cup of Hockey in September 2016.