Colligiana only played in the amateur leagues, playing in over twenty Serie D championships (known by different names over the years, including Second Division, Third Division Interregional, IV series, National Amateur Championship and League Inter [2]) until the 2008–09 season when it advanced to Lega Pro Seconda Divisione B.

Under the chairmanship of Englishman Justin Gallant and coach Gino Manni, Colligiana set an Italian and European record of 17 consecutive wins, becoming Tuscan first division champion and making national television and newspapers.

In May 1974 Colligiana defeated Serie A club Genoa 2–1 in a friendly match, facing star players Mario Corso, Robert Rosato, Luigi Simoni, Claudio Maselli and Antonio Bordon.

To return to its former glory, Colligiana hoped to rely on local players like Paolo Fratiglioni (1968–69 goalkeeper), Franco Michelucci, Paolo Buzzichelli, and Gaetano Siracusa, as well as some younger players like Luca Vettori, Carlo Pasquale Pondrelli Massimo Conforti, "Gigino" Gozzi and the promising but frequently-injured Sergio Secchi.

Players included goalkeepers Filippo Gambelli and Andrea Alboni; defenders Gino Balli, Alessandro Caioni, Massimo Cardelli, Riccardo Roselli, Giovanni Guerrini, Simone Settesoldi, Baldassarre Buzzichelli and Fabio Giotti; midfielders Nazzareno Baggiarini, Federico Frediani, Daniele Visani, Cristiano Signorini, Simone Baldi, Isaia Tani and Damiano Baldini; and attackers Antonio Bruno, Giovanni Giordani, Alessandro Franchi and Stefano Cipriani.

Colliagiana finished behind Pro Vercelli, despite prolific striker Mastacchi's 19 goals and Filippo Gambelli on defence going 7 games without conceding.

Colliagiana ranked fourth last to place and despite appeals for irregularity in the match lost to Todi, was demoted to the Eccellenza league.

Many players from the 1980s and 1990s have returned to Colligiana in recent years, including strikers Claudio Mastacchi and Massimiliano Santoro, Filippo Gambelli, Gianni Agrumi, Simone Baldi, Franco Baldini, Mario Boschi, Alessandro Caioni, Marco Calonaci, Fabrizio Calattini, Massimo Cardelli, Alessandro Cianetti, Graziano De Luca, Claudio Di Prete, Sauro Fattori, Giancarlo Favarin, Federico Frediani, Gabriele Garfagnini, Giovanni Guerrini, Mattia Masi, Ruby Mearini, Franco Merendi, Tommaso Movilli, Massimiliano Pellegrini, Riccardo Roselli, Sergio Sezzatini, Cristiano Signorini, Giuseppe Sacchini, Marcello Sansonetti, Luca Tognozzi, Daniele Visani, Giacomo Zanelli, and Eugenio Zangrillo.

The club was coached by Mr. Roberto Biccherai and players included goalkeepers Lapo Giusti, Marco Gioli and Francesco Bianciardi; defenders Gionata Antoni, Davide Bertolucci, Luca Bonini, Massimiliano Burchi, Marcello Labruna, Andra Pobega and Alessio Vannini; midfielders Hector Simon Acosta, Paolo Andreotti, Alessandro Bartoli, Andrea Corsi, Stefano Illiano and Matteo Lombardi; and attackers Luigi Guerrera, Manuel Nencini, Atos Rigucci, Fermin Rojas, Dario Pietro Tranchitella and Simone Vespignani.

In the same season the junior club led by Paolo Molfese won the Championship Class and the title of regional champion.

In the 2008–2009 season, Colligiana's sole goal was to stay in the Lega Pro Seconda Divisione under the leadership of Roberto Bicchierai and Nario Cardini.

Colligiana then played Biancorssa Giacomense in play-out that resulted in two draws and exclusion from Serie D. In the 2010–11 season, the club was restarted from Terza Categoria Siena with the new name A.S.D.

[1] The sports complex is located in Via Liguria, in the Badia area, and is dedicated to Gino Manni, the player of the late thirties and early fifties.

Organized support for Colligiana began in September 1977, thanks to Team "Guelfi Colle", which led fan efforts until 1983.

The most recent friendly match between the clubs took place 20 August 2006 with a 2–0 Colligiana victory led by goals from Alessandro Bartoli and Atos Rigucci.

In the 2009–10 season the Second Division Pro League derby home leg was particularly exciting, with fewer than two goals scored by half time.

The historic rivalry with Siena derives from an old alliance between Florence and Colle di Val d'Elsa at the time of the Guelfi and ghibellini, where the two opposing factions fought in the 1269 Battle of Hill of 1269, won by Guelfa.

The rivalry heated up in the summer of 2000, when the city council decided to remove the lilies from a particular patch, unleashing the wrath of many Colligiana fans.