Flying UH-34Ds, the squadron's mission was to provide assault transport of troops and cargo in support of Fleet Marine Forces during ship-to-shore movements.

HMM-365 was reassigned to Marine Corps Air Station Futenma, Okinawa in August 1964 in preparation for the squadron's deployment to the Republic of Vietnam.

The squadron arrived at Da Nang Air Base on 7 October 1964 and immediately began performing re-supply missions in support of Operation Shufly.

On 11 October, the newly arrived Marine pilots and aircrews were involved in their first firefight when 8 UH-34Ds drew Viet Cong fire while landing a 112-man Vietnamese unit in the hills 10 miles (20 km) west-southwest of Tam Ky. On 26 October, the Blue Knights suffered their first combat casualties when a copilot and crew chief were wounded by Viet Cong small arms fire while their helicopter was approaching an enemy contested landing zone 10 miles (20 km) southwest of Tam Ky.

When flight operations resumed on 10 November, the crews concentrated on rescuing Vietnamese civilians from the inundated coastal plains.

Many of the rescues were accomplished by hoisting individuals from precarious positions in trees or on rooftops while being subjected to sporadic Viet Cong harassing fire.

The TK-1, an externally mounted combination of M-60 machine guns and 2.75-inch (70 mm) rocket launchers, that was first used in support of a Tiger Flight mission conducted just south of the Song Thu Bon, about 17 miles (27 km) from Da Nang.

On 6 January 1991, while en route to Iraq, HMM-365 and HMM-263 conducted Operation Eastern Exit, the Non-combatant Evacuation (NEO) of the American Embassy in Mogadishu, Somalia.

On 26 February the Blue Knights conducted a night time, long-range mission in support of the amphibious demonstration against Bubiyan Island.

After securing both Camp Rhino and Kandahar Airport deep in Afghanistan, the Blue Knights supported coalition forces conducting raids against the Taliban from December 2001 to February 2002.

In March, the ARG transited to the Red Sea for a potential NEO in Yemen and to support Vice President Dick Cheney’s visit to the region.

In January 2003, the Blue Knights prepared for deployment to Ali Al Salem Airbase, Kuwait in support of Operation Iraqi Freedom (OIF).

OIF began on 20 March, with HMM-365 leading the TRAP Team effort for the initial heliborne raids on the crucial Al Faw Peninsula.

Blue Knight crews flew multiple raids and recon inserts as ground forces maneuvered north towards Baghdad.

Due to the expeditious nature of I MEF's battle rhythm, section detachments were configured into combat crews and pushed forward with coalition forces in order to provide 24-hour support.

Blue Knight aircrews flew 200 to 300-mile (500 km) missions across the Kuwaiti border, into Iraq, to conduct relief operations with other sections for up to a week at a time.

The squadron supported I MEF operations from Ali Al Salem until mid-April when it re-deployed itself in a single day to Jalibah FOB, Iraq where it rejoined MAG-29.

On 23 February 2005, HMM-365 returned to CONUS after flying more than 3,961 combat hours, 2,404 sorties, 11,162 passengers, and 748,496 pounds of cargo without the loss of aircraft or personnel.

The MEU was subsequently sent into Afghanistan as part of Operation Enduring Freedom where their aircraft have been spread out and based out of Kandahar Airfield and Camps Bastion and Dwyer which are over 100 miles away.

The squadron expanded the accepted capabilities of the MV-22 by operating across the spectrum of Assault Support and displaying wide-ranging and flexible skill sets.

The maintenance department's Emergency Reclamation Team was tested on several occasions; to include, 27 June 2012 when two separate aircraft received significant battle damage after inserting 1st Reconnaissance Battalion Marines to a tactical landing zone.

One of these aircraft was forced to make an emergency landing to a nearby FOB after taking 12 enemy medium machine gun rounds.