Sir Valentine Knightley (c. 1555 – 9 December 1618) was an English landowner and Member of Parliament.
He was the eldest son of Sir Richard Knightley of Fawsley Hall, Northamptonshire and educated at Hart Hall, Oxford (1568), where he was awarded MA in 1605 and trained for the law at Gray's Inn (1583).
He served as a Justice of the Peace (J.P.) for Northamptonshire at various times and as a judge of assize in the oyer and terminer courts on the Oxford circuit from 1609 to his death and on the Midland circuit from 1616 to his death.
He was appointed High Sheriff of Berkshire for 1617–18 and was a deputy lieutenant for Northamptonshire by 1618.
The Fawsley estate passed to his nephew Richard, also an MP for Northamptonshire.