Valentine National Wildlife Refuge is located in the U.S. state of Nebraska and includes 19,131 acres (77.42 km2).

The refuge protects a portion of the largest remaining area of tall and mid grass prairie in the United States.

Collectively known as the sand hills region of Nebraska, the dunes were the result of the last ice age known as the Pinedale glaciation.

During the Holocene glacial retreat the sand dunes that been deposited in their current location by the vast continental glaciers, were exposed and grasses eventually took over.

Coyote, blanding's turtle, prairie grouse, white-tailed and Mule deer are commonly found by visitors in this refuge.