It was located at Vallø Castle just south of Køge on the east coast of the island of Zealand.
It functioned as a convent for unmarried women from noble or princely houses, who resided at Vallø Castle.
For this purpose, Vallø Castle was expanded between 1736-38 with a new baroque-style building designed by architect Lauritz de Thurah (1706–1759).
The abbess had the right to appoint the vicars in the parishes belonging to the county of the stift.
In 1976, the foundation's bylaws were revised so that no more new ladies of the diocese were to be enrolled, but noble widows who were already registered could get free housing in the castle.