It runs through the valley where there are many chicken farms and was therefore originally called the Kippenlijn until a project was launched on 10 December 2006.

From there the line is 17 km long and takes 20 minutes to operate from Barneveld Noord to Ede-Wageningen.

The following stations are on or serviced by the Valleilijn (Hoevelaken is technically situated on the Oosterspoorweg), including some possible interchanges: Driedorp, Appel, Dusschoten, Voorthuizen, Barneveld Kruispunt, Meulunteren, Doesburgerbuurt, Stompekamp and Ede Gemeentehuis.

Before 10 December 2006, the line was operated by Nederlandse Spoorwegen but on that date, Connexxion took over the service and made a lot of improvements.

From 12 December 2022 transdev took over the rrreis bus services due to the company being accused of fraudulent activities.