Venus traveled to New York, where she encountered the Teenage Mutant Ninja Turtles watching over the spiritless body of their master.

After helping the turtles put an end to Shredder and the Foot Clan, she led them on a dream walk to rescue their master's spirit.

While proficient in fighting techniques, Venus was not trained in Ninjutsu like her brothers and thus, she would often use mystical orbs in battle (to various degrees of success).

This was done by the writers so as not to eliminate the possibility of a romantic relationship between Venus and one of the four male turtles, with hints leading primarily to Raphael and Leonardo.

After the cancellation of the show, an informal "second season" was formed on the franchise's official website, consisting of letters "written by" each of the turtles (the site has since been removed because of the sale to Viacom).

[8] This incarnation started as a Frankenstein-like creature created by the mad mutant surgeon Doctor Jasper Barlow from the remains of a female Punk Frog named Bonnie.

Later, when Donatello attempts to stop Armaggon, whom he had unwittingly unleashed in the first place, from eliminating the Turtles from the timestream, Venus accompanies him into the past and the future.

After a turbulent journey and encounters with their past and future selves, they realize that Armaggon's creation and rampage is an inevitable aspect of the Turtles' reincarnation from their human selves in feudal Japan to their present mutant forms.

In order to close this circle, Venus sacrifices her life to Armaggon in Donatello's place; her spirit later briefly returns to the mortal world in the past to infuse the then-unmutated Turtles and Splinter with the quantum-temporal impressions (called "quantumnucleid acid") their existence has left in the timestream, thus triggering their rebirth.

[10] During an interview, Kevin Munroe, director of the TMNT animated film, elaborated on Peter Laird's instructions saying that, "There's absolutely no mention of Venus de Milo, the female Turtle.