Vlastiboř is a municipality and village in Tábor District in the South Bohemian Region of the Czech Republic.

Vlastiboř consists of three municipal parts (in brackets population according to the 2021 census):[2] The name is derived from the personal name Vlastibor, meaning "Vlastibor's (court, property)".

It lies mostly in the Třeboň Basin, but it also extends to the Tábor Uplands in the north and east.

The stream Bechyňský potok flows through the municipality.

There is a set of fishponds fed by the stream, situated in the northern part of the municipal territory.