He was owner of the Poturzyca, Zarzecze, Kramarzowka, Markpol, Lachowice, Dobraczyn, Medowa, Jaryszow, Konarzewo, Gluszyn, Wiry and Szczytnik estates and a founder of the Natural History Museum in Lviv (Lwów).

Dzieduszycki was born in Jaryszów in the Russian Podolia, the son of Józef Kalasanty (1776–1847) and Paulina Anna née Dżialyńscy and was educated in Poland and from 1840 in Göttingen and Paris.

Dzieduszycki collected specimens of fauna and flora from his childhood and held them in his palace in Poturzyce, 80 km north of Lviv.

[7] He was also co-founder of the Museum of Industry of Arts (Muzeum Przemysłu Artystycznego) in 1874 in Lviv and founder the "Hunting Society of Lesser Poland" in 1885.

Włodzimierz married Alfonsyna Miączyńska on 19 September 1853 in Lwów and had four children: Dzieduszyckia, a genus of Devonian brachiopods, was named in his honour.