Du Bois High School served students in Baltimore City, grades 9 through 12.

It had two Career and Technology Education Pathways of Agricultural & environmental science and Project Lead the Way (PTLW).

The Du Bois squad won the Rookie Inspiration Award at a competition in Annapolis, Maryland, in March 2006 and was invited to demonstrate in Chicago, Illinois, summer 2006, in front of the National Technical Association.

The 2008 Panthers Football team for Du Bois won its first playoff game in school history against rival Reginald F. Lewis, 9–7.

[6] The team then lost to Paul Laurence Dunbar High School, 46–0, in the second round of the state playoffs, ending their season.