WRUF is a sports station that covers University of Florida athletics.

WRUF featured a mixture of local and syndicated programs, including Jim Bohannon, Dr. Joy Browne, Larry King Live and Sporting News Radio, plus religious programming on Sunday mornings, including Bill Gaither, The Director of Programming is Rob Harder, Assistant Program Director/Brand Manager is Seth Harp and the Sports Director is Steve Russell.

WRUF's sports news departments, staffed almost entirely by School of Journalism students, are fairly large for a station of its size; by at least one account it has the largest local radio news department in Florida.

A WRUF microphone used by UF alumnus Red Barber during the 1930s is part of the National Baseball Hall of Fame and Museum's collection.

It exists mainly to fill in the gaps because Tower #3 of their directional AM array failed in 2016, and the FCC granted permission to operate non-directionally after sunset at 25% of licensed power.