Waldbüttelbrunn is a municipality in the district of Würzburg in Lower Franconia, Bavaria, Germany.

Archaeological excavations have shown that the area was settled as early as 5000 BC.

The first official document mentioning "Waldbüttelbrunn" as "Büttelbrunn" dates from AD 748.

One of the last battles of the Austro-Prussian War of 1866 took place at Rossbrunn, now part of Waldbüttelbrunn, between Bavarian and Prussian troops.

Waldbütellbrunn is in good contact with the French municipality of Fleury sur Orne in Normandy, with annual visits and student exchanges.