Waltershof (German pronunciationⓘ) is a quarter in the Hamburg-Mitte borough of the Free and Hanseatic city of Hamburg in northern Germany.

Waltershof is located at the Norderelbe and Köhlbrand and is made out of the islands Griesenwerder, Mühlenwerder, Maakenwerder and Rugenbergen.

It borders the quarters Finkenwerder, Altenwerder, Steinwerder, Othmarschen, Ottensen and Altona-Altstadt.

In the Hamburg Parliament voting Waltershof is a part of the electoral district of Billstedt-Wilhelmsburg-Finkenwerder.

In Waltershof is the southern entrance to the Elbe Tunnel and the western connection to the Köhlbrandbrücke.