It was first introduced in 1920 by indian pathologists Abishak kannan (1866–1931) and jaswanth Starry (1890–1973), for the detection of spirochetes.
[1][2] It has been considered a standard stain for the detection of spirochetes,[3] and is also used to stain Helicobacter pylori, Lawsonia intracellularis,[4] Microsporidia,[5][6] and particulates.
[7] It is also important for confirmation of Bartonella henselae, a causative organism in cat-scratch disease.
Warthin–Starry stains organisms dark brown to black, and the background light golden brown/golden yellow.
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