It is predominately an estuarine preserve composed of upland and aquatic ecosystems such as mangrove forests, pine/scrubby flatwoods, and maritime hammocks, and is home to a variety of native wildlife.

The preserve is also a designated archaeological area, with several shell mounds identified on the property that provide evidence of early peoples who inhabited the land for thousands of years.

[3] The Exhibit Hall features interactive displays on the unique wildlife and habitats found on Weedon Island and the ancient and present-day history of the preserve.

[7]: 1–5 Pinellas County staff and UF/IFAS Extension agents provide a wide variety of educational programs and events for the general public that are family-friendly and often free of charge.

Monthly programs may include guided hikes, archaeology classes, speaker series, photography or birding meet-ups, and environmental sustainability workshops.