In the east, it is patchily distributed from the Free State in South Africa to Ethiopia in the north, while further west it is also found in Zambia, the southern Democratic Republic of the Congo, and central Angola.

[1] Within this large region, the bat has been reported form a range of different habitats, but appears most common in open woodland and savannah.

Welwitsch's bats generally roost alone, in trees, low bushes, artificial structures, or deep caves.

Its diet consists of aerial insect prey such as moths, beetles and bugs, based on limited observations in Mpumalanga, South Africa.

Other sympatric species of Myotis bats tend to be seasonal breeders, with mating observed from late spring to summer and normally rearing between one and two young.