The source of West End Brook is from springs near Strawberry Farm, to the north of Tadley and south of the Atomic Weapons Establishment at the former airfield of RAF Aldermaston.

The springs are just above the 330-foot (100 m) contour, to the north of the Hampshire county boundary in Berkshire,[1] and very close to West's Meadow, Aldermaston Site of Special Scientific Interest (SSSI), 2.96 acres (1.20 ha) of unimproved meadows which are unusual, as the well-drained sandy soils remain damp, probably due to a layer of clay material beneath them.

[6] A stream flowing from two springs on Benyon's Inclosure joins on the left bank as it passes under Church Road, at which point it has already descended to 240 feet (73 m) Above Ordnance Datum (AOD).

The course of another Roman road crosses at Lovegrove's Farm and it is joined by another stream, draining from Hundred Acre Piece, a large area of woodland to the north.

Water quality on West End Brook dropped from moderate to bad in 2015, and the reasons for it not being good are mainly to do with physical modification of the channel, which prevents fish freely moving around the system, and the presence of the invasive species, the North American signal crayfish.