[6] Some of his more notable roles include: as Dr. Allen in Marshal of Laredo (1945), one of the series of Red Ryder films;[7] as Lewis Havener in the 1946 fantasy-horror film The Flying Serpent;[8] as Jasper Braydon in Stagecoach to Denver, a 1946 western directed by R. G. Springsteen;[9] as Doctor William R. James in the 1951 film noir The Prowler, starring Van Heflin;[10] as Sam Wilkins in the 1952 western Wagons West, starring Rod Cameron;[11] and as Doc Runyon in the western The Peacemaker (1956).

The serials in which he appeared include: as Dr. Humphrey in Drums of Fu Manchu (1940); as Boswell in Adventures of Red Ryder (1940), as Professor Benjamin in The Purple Monster Strikes (1945); as Wilson in The Crimson Ghost (1946); and in the featured role of Caleb Baldwin in Son of Zorro (1947).

Chambers' debut on the small screen was in the featured role of Father Batista in the eighth episode of the classic television western, The Lone Ranger, in 1949.

Chambers' last performance was in a small role in the 1958 film, Gunman's Walk, starring Van Heflin and Tab Hunter.

Chambers had worked on the film in December 1957, and it was released in July, 1958, six months after his death.