He has played for Queensland in the State of Origin series, Prime Minister's XIII and the Indigenous All Stars.

Chambers was born in Brisbane, Queensland, Australia[4] into a family of Indigenous Australian (Yolngu)[5] descent but moved to the remote Northern Territory town of Nhulunbuy at 2 years of age.

[citation needed] He began playing junior football for Nhulunbuy in the Northern Territory Rugby League competition.

[6] At the age of 13, he returned to Brisbane and played schoolboy football for St Joseph's College, Nudgee.

By being part of the 2012 NRL Grand Final winning team, Chambers joined Peter Ryan, Brad Thorn and Sonny Bill Williams in winning both NRL and Super Rugby titles (though he did not play in the Super Rugby final).

[17] Chambers switched to rugby union from 2010 after signing a 2-year deal with the Queensland Reds to play in the Super 14.

[18] However, following a disappointing 2011 season - where he only started six of the Reds' 18 matches - Chambers was released by the club after missing a place in their thirty-man 2012 squad.

[19] He was named on the bench for the Reds Super 15 final victory over the Crusaders in 2011 – which featured fellow league-turned-union player Sonny Bill Williams on the opposing side – but did not play in the match.

[27] This was announced to be Chambers last appearance for Munster, as he was returning to Australia to take up a contract with rugby league team Melbourne Storm.

Following the conclusion of the 2019 Melbourne Storm season, Chambers was honoured at the club's annual awards night as one of the departing players, confirming rumours that he would be leaving the NRL.

[30] Chambers would later be unveiled by Suntory Sungoliath, who also had former Queensland Reds Sam Talakai, Hendrik Tui & Samu Kerevi on their roster.