He was the second eldest son of Alexander Sutherland, 8th of Duffus and his wife Janet, daughter of James Grant of Freuchie.
[1] In 1579, he was infeft in the lands of Duffus and Greschip, near Elgin, Moray, as heir of his father, the deceased Alexander Sutherland.
He also received formal possession of Quarrelwood which had belonged to his grandfather, William Sutherland, 7th of Duffus who had died in 1543.
[2] At the instance of William Sutherland, 9th of Duffus, a commission was granted to the Earl of Sutherland along with Robert Munro, 18th Baron of Foulis and others on 15 March 1614, for them to apprehend three men who had murdered a certain Donald Angus Gairson and who had failed to appear before the Justice on the day appointed to answer the charge against them.
[2] William Sutherland, 9th of Duffus married firstly, Margaret, daughter of George Sinclair, 4th Earl of Caithness.