He was articled to David Laing, architect of the new Custom House, and surveyor to the Parish of St Dunstan-in-the-East.

[2] In 1827–8 Tite built the Scottish church in Regent Square, St Pancras, London, for Edward Irving,[3] in a Gothic Revival style, partly inspired by York Minster,[4] and ten years later collaborated with Charles Robert Cockerell in designing the London & Westminster Bank head office in Lothbury, also in the city.

Tite's winning design has an imposing eight-column entrance portico, inspired by the Pantheon in Rome, while the other sides of the building are based on Italian renaissance models.

The refreshment rooms had "an open timber roof, and oriels or bays, reminiscent of the dining-hall of olden times".

[3] Tite's active work as an architect ceased about twenty years before his death[3] (in recognition of his contributions, however, he was awarded the RIBA Royal Gold Medal in 1856).

[2][3] He was also a Governor of St. Thomas's Hospital, London, where he is commemorated by the William Tite Scholarship, for the best student in the first year, with the highest aggregate marks in Anatomy and Physiology.

After over 125 years, this prize has been subsumed into King's College London,[10] where it is still awarded for excellence in the pre-clinical medical course.