The most well-known include Mickey's girlfriend Minnie, pet dog Pluto, friends Goofy, Horace Horsecollar, Clarabelle Cow, and nemesis Pete.

[12]) Mouseton's location in Calisota and its positioning with regard to Duckburg (the cities being not far from one another) were the subject of speculation early on, but have generally been treated consistently in American publications from 2003 onward.

Mouse Takes a Trip (1940) Mickey and Pluto live in the actual world city of Burbank, California, home of Walt Disney Studios.

This is a reference to the fact that Oswald was Walt Disney's primary cartoon star before the creation of Mickey Mouse, though he was owned by Universal Pictures at the time.

Kat Nipp reappeared in a 1931 sequence of the Mickey Mouse newspaper comic strip (in which his friend Barnacle Bill, a sailor who is an expert in untying knots, appears).

Squinch returned in several more storylines: originally depicted as an unscrupulous businessman, he evolved into an all-out criminal, playing alongside Pete a role similar to Sylvester Shyster's in earlier strips.

He was later used by Italian authors, starting with the 1959 story Topolino e il ritorno dell'artiglio magnetico ("Mickey Mouse and the Return of the Magnetic Claw") by Guido Martina (plot) and Giulio Chierchini (art).

In Who Framed Roger Rabbit (1988), five Weasels partially based on those of "The Wind in the Willows", with different appearances and personalities, form the so-called "Toon Patrol", acting as the secondary antagonists of the story under the service of Judge Doom.

In the video game Mickey Saves the Day, several Weasels appear as Pete's henchmen in his plan to become mayor of the town, working for him as policemen or security guards.

In the latter universe, he is a recurring antagonist for Mickey Mouse and, in particular, for Goofy in his Super Goof incarnation, in which case Emil Eagle becomes a kind of Lex Luthor.

"Dangerous" Dan McBoo and Idgit (sometimes spelled "Idjit") "the Midget" are a pair of criminals created by Paul Murry in the 1966 comics story Treasure of Oomba Loomba.

He is so manipulative and charismatic that he managed to fool the entirety of Mouseton into thinking he was a good guy more than once, with Mickey usually being the only one skeptical of him (according to his debut story "The Magnificent Doublejoke", they were schoolmates until Charlie's habits of bullying others had gone so far that he was expelled from school).

Gideon is married to a female anthropomorphic goat named Gertie (presumably Gertrude) who appeared in many early Mickey Mouse comics, primarily as a background character.

In one episode of that series, "Double Date Don", she fell in love with Donald Duck and aggressively pursued him by puckering her lips in front of him, forcing him to dance with her, wearing dresses and posing provocatively to lure him in.

Doctor Einmug specializes in atomic physics and speaks in a German-like accent which was probably a nod towards Albert Einstein, "mug" also being a pun on "stein".

He was created by Floyd Gottfredson (art) and Bill Walsh (plot) and first appeared on September 26, 1947, in the Mickey Mouse comic strip storyline The Man of Tomorrow.

The complete original run of Eega Beeva stories has been anthologised in The Floyd Gottfredson Library volumes 9 Rise of the Rhyming Man and 10 Planet of Faceless Foes, published by Fantagraphics Books in 2016.

He eats pickled kumquats for food (changed to mothballs in Italian translations and stories produced in Italy) and is severely allergic to cash; these traits have sometimes been used as plot devices.

On the other hand, Ellsworth is also a bonafide genius with awesome tech and scientific knowledge—the "Y" on his shirt in earlier stories stands for "Yarvard" (a parody of Harvard), his alma mater.

Despite being more or less entirely humanized in more recent stories, Ellsworth retains his ability to fly, a unique trait among the central Disney funny animal cast.

[44] In his appearance as well as his role in the stories, Atomo is very similar to Gottfredson's Eega Beeva, a short, friendly science-fiction character with unpredictable powers that drive the plot.

She was first created by Bill Walsh, and appeared in some "Mickey Mouse" dailies by Floyd Gottfredson, and others written by Del Connell (drawn by Manuel Gonzales).

The Sleuth is a good-natured gentleman; wearing a deerstalker hat, smoking a pipe and using a magnifying glass, he is an obvious parody of Sherlock Holmes, Mickey basically playing the part of Dr. Watson.

Nevertheless, he always manages to solve his cases – hence ensuring a reputation as a great investigator – either by sheer luck, or thanks to his foes' fecklessness or simply because Mickey Mouse does all the actual detective work for him.

The characters of The Sleuth and Professor Nefarious, complete with the University of Criminal Sciences and the henchman Fliplip, were portrayed in an extended sketch in an episode of The New Mickey Mouse Club in 1978.

He first appeared in the story La lunga notte del commissario Manetta (English title: Casey's Longest Night) in 1997, written by Tito Faraci and drawn by Giorgio Cavazzano .

Ever since the end of "Shadow of the Colossus", she has been obsessed with Atlantis; in her quest for the lost continent, she has repeatedly confronted a secret society called "Horde of the Violet Hare" (also created by Casty), who want to use Atlantean technology for their own goals.

In the stop motion TV special Mickey Saves Christmas (2022), Cuckoo-Loca makes a cameo appearance, with a figurine of her emerging from the cuckoo clock in Santa's workshop.

In the video game Kingdom Hearts III (2019), Beppo appears as an enemy in the "Mickey's Mechanical Man" minigame, based on in the short film of the same name.

Storyboard sketches of a planned cartoon featuring Bobo, titled Spring Cleaning, were printed in the book Mickey Mouse: The Floyd Gottfredson Library – Volume 3: Showdown at Inferno Gulch.