The varsity teams compete in the Ontario University Athletics provincial conference and the national U Sports organization.

The program is notable for featuring three Hec Crighton Trophy winners, most recently being former Canadian Football League running back, Daryl Stephenson, when he won the award in 2006.

In 2011–12, Coach Vallée and the Lancer women's basketball team captured their second straight CIS national title with a 69–53 over the second seeded UBC Thunderbirds.

Ranked fourth heading into the national tournament, the Lancers defended their 2011 title by defeating AUS champion Acadia in the quarter-final, host Calgary in the semi, and the Canada West winner Thunderbirds to take the Bronze Baby back to Windsor.

In 2012–13, Vallée's squad made program history as they completed an undefeated regular season with a 21–0 record and the No.

In 2013–14, Coach Vallée led her team to an incredible fourth straight CIS national championship title, winning at home for the second time in four years.

The Lancer track and field program has featured a number of high-profile athletes over the years including national standouts O'Brian Gibbons, Andrea Steen, Mike Nolan, Ryan McKenzie and most recently Melissa Bishop.

Bishop, who is still coached by Dennis Fairall, won a gold medal at the 2015 Pan Am Games in Toronto, Ontario.

She also set a new Canadian record in the 800 metres in the summer of 2015 while also capturing a silver medal at the 2015 IAAF World Championships in Beijing, China.