[1] Located in southeast Wisconsin, the district comprises parts of western Milwaukee County.

It includes most of the city of West Allis and parts of western Milwaukee and southwest Wauwatosa.

The 2002 court-ordered redistricting was the first to extend the district into Waukesha County, when the district was drawn to comprise the eastern half of the city of Brookfield with the village of Elm Grove, in Waukesha County, along with the western half of the city of Wauwatosa and western wards of the cities of West Allis and Milwaukee, in Milwaukee County.

[7] The 2022 redistricting sought to cram more Democratic votes into this district in order to make the 13th Assembly district a more safely Republican seat, so all the Waukesha County wards were removed in favor of more of Milwaukee County.

Notable past representatives of the 14th Assembly district include Tom Barrett, the 44th mayor of Milwaukee, who also served in the United States House of Representatives, representing Wisconsin's 5th congressional district for five terms, and Scott Walker, who became the 45th governor of Wisconsin and was a candidate for President in the 2016 Republican Party presidential primaries.