It connected with what is now the Florida Northern Railroad in Citrus Springs with what is now CSX's Wildwood Subdivision (S Line) in Owensboro (a train junction, south of Trilby).

The segment was officially declared as the DuPont—Lakeland Line, part of a passenger rail route that afforded a more direct path from Georgia to Gulf Coast tourist destinations.

The trail runs under I-75 as it continues north and about a mile from there intersects the Good Neighbor Trail at 28°35'22.2"N 82°13'42.3"W. From there it runs through Nobleton, Istachatta, and then crosses the Hernando-Citrus County Line into Pineola, the site of the Great Train Wreck of 1956.

Beneath the bridge under US 41, another trail leads to Whispering Pines Park, and from there it makes a right curve around White Lake.

Just south of the intersection with East Arlington Street, it runs relatively close to the west side of US 41.

In Hernando, the trail is briefly detoured around County Road 486 for one block, then resumes its use of the former railroad right-of-way.

Before reaching Holder, it has an intersection with another trail leading to the Central Ridge District Park.