With the support of his regent, his uncle Rupert (later made the Count of Veldenz), Wolfgang introduced the Reformation to Zweibrücken in 1537.

His father died in 1532, so the regency of Palatinate-Zweibrücken passed to Louis' younger brother Rupert until 1543.

In 1548 the Holy Roman Emperor Charles V occupied his Protestant territories and reintroduced Catholic practices.

When the young Wolfgang's father died, his uncle Rupert had served as the child's regent.

In 1543, when Wolfgang reached majority and took on the responsibility of office, he enacted the Marburg Contract, giving Rupert the County of Veldenz.