Born in Hunan, China in 1908, he moved to Suzhou at the age of four with his family, where he lived for the rest of his life until his death in 1997.
Raised in a musical family, he learned the guqin from his father, and in 1921 became a student of Wu Jinyang.
Wu Zhaoji is popularly revered as an archetypal "literatus" qin player; his smooth, detached, intellectual, yet vigorous style made him one of the most highly regarded amateur players in the late 20th century.
Though a well-known Guqin master, Wu was, by profession, a mathematics professor at Soochow University in Suzhou, Jiangsu Province.
The "Wu" school of qin playing currently centered in Suzhou takes him as a leading figure; noted players to transmit his style include Wang Duo and Yuan Jung-ping.