Yeehaw Junction is a census-designated place (CDP) in Osceola County, Florida, United States.

[15][16] In late 1968 the Deseret Test Center conducted a biological warfare experiment at Yeehaw Junction.

Stem rust, referred to as "Agent TX", was tested to determine its effectiveness against a wheat crop in time of war.

The racial and ethnic composition of the population was 89.2% non-Hispanic white, 1.3% Native American, 0.4% Asian (one person), 2.9% reporting two or more races and 6.3% Hispanic or Latino.

It is located at the southern end of the longest stretch of limited-access highway without an exit in the United States (the next interchange to the north being 48.9 miles away at Kissimmee/St.

Cloud) and the northern end of the second-longest such stretch (the next exit to the south being 40.5 miles away at Fort Pierce).

[23] The Desert Inn was largely destroyed on December 22, 2019, when an 18-wheeler lost control on a nearby road and crashed into the building at highway speeds.