[3] Prior to enrollment as a cosmonaut, Serova had worked as an engineer of the 2nd category for the RSC Energia, and in the Mission Control Center.

[3] Serova was selected as a test cosmonaut at the age of 30 in the RKKE-14 group in October 2006 while working as a flight engineer.

[8] The Soyuz FG rocket carrying TMA-14M commander Aleksandr Samokutyayev, Yelena Serova and NASA astronaut Barry Wilmore blasted off from the Baikonur Cosmodrome's Site 1/5 at 20:25 UTC.

When leak checks were complete, the hatch on the Poisk module was opened at 5:06 UTC and Serova with Samokutyayev and Wilmore entered the Space Station.

Speaking at the plenary meeting of the Parliamentary Assembly of the Organization for Security and Co-operation in Europe on 8 July 2019, she stated that "On the board of the International Space Station, [she and other astronauts] had a chance to see with naked eyes how bombs and shells exploded in Donbass and Luhansk.

[citation needed] In July 2017 Serova was a vice chairperson at a meeting to discuss a bill on the protection of animals.

[13] Serova was one of five cosmonauts selected to raise the Russian flag at the 2014 Winter Olympics opening ceremony in Sochi.