The Yolobus fleet consists of 50 compressed natural gas (CNG) buses and 10 paratransit vehicles.

The following cities are served: Sacramento, West Sacramento, Southport, Davis, Woodland, Cache Creek, Madison, Esparto, Capay, Knights Landing, Dunnigan, Yolo, Vacaville.

Commute and express routes run only at peak times in the mornings and evenings, Monday-Friday.

BeeLine On Demand Transit was also launched in June 2023 to replace lost service for trips within the city of Woodland.

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