Zachary Brooke (1716–1788) was an English clergyman and academic, Lady Margaret's Professor of Divinity at the University of Cambridge.
He was educated at Stamford school, and was admitted a sizar of St. John's College, Cambridge, 28 June 1734.
He was elected to the Margaret professorship of divinity at Cambridge in 1765, and was at the same time a candidate for the mastership of St. John's College.
He was chaplain to the king from 1758, and was vicar of Ickleton, Cambridgeshire, and rector of Forncett St. Mary and St. Peter, Suffolk.
He attacked the Free Inquiry of Conyers Middleton in his Defensio miraculorum quæ in ecclesia christiana facta esse perhibentur post tempora Apostolorum, Cambridge, 1748, which appeared in English in 1750.