Zelotes subterraneus is a species of ground spider from the family Gnaphosidae which has a Palearctic distribution.

[3] Zelotes subterraneus is one of a group shiny, all-black Zelotes species which are difficult to identify,[2] the females being extremely similar and the males only being readily identifiable by reference to the tip of the embolus on the palps.

[5] Elsewhere in Europe habitats utilised include woodland, heathland, dry meadows, boggy areas, under stones and bark and in the litter layer, up to 1300 m above sea level.

The white egg sac is attached to the underside of stones and guarded by the female.

[7] It is an active, nocturnal hunter, running down and catching a variety of terrestrial arthropods including other species of small spiders.