The plot is a fairly standard western storyline about a villain attempting to illicitly take valuable land (in this case a new railroad).

Carroll's "best moments" in costume were singing (Lyrics include "Zorro rides again into the night...")[2] It was shot in Cochilla, Mexico[3] and featured other locales such as Bronson Canyon, Iverson Movie Ranch, Red Rock Canyon State Park, Angeles National Forest, and Chatsworth, Los Angeles.

In the opinion of Cline, one of the most memorable stunt scenes in the history of film serials is shown in Zorro Rides Again.

Stuntman Yakima Canutt plays Zorro as he gallops up to the cab of a moving truck and swings from the saddle to its running board.

The feature film had a working title of Mysterious Don Miguel before returning to the original name Zorro Rides Again.