The male has distinctive enlarged chelicerae (the mouthparts used for grasping prey) and front femora (the third, and typically largest, leg segments).

[4] Hentz named the species Attus sexpunctatus and described it as follows:[5] "Black; cephalothorax with the two posterior eyes near the base, which is wide and suddenly inclined at nearly a right angle with the upper surface, cheliceres with a strong inner tooth, and a long, curved fang; abdomen with six dots, and a line in front, white; feet, 1.

[5] The legs are reddish brown, or sometimes yellowish,[10] with the femora of the anterior (first) pair being darker and enlarged, especially in the male.

[3] The range of the species extends from New Jersey to Florida and west to Texas,[15] although it is most commonly found in the southern states.

[5] In 1909, the Peckhams reported that the species had been collected from North Carolina, Florida, Texas, Louisiana, and Mississippi.

[10] A seven-year survey of spider species in western Mississippi reported the abundance of Z. sexpunctatus as "uncommon".

[23] Robert and Betty Barnes reported the species as occurring in broomsedge fields throughout the southeastern Piedmont.

[24] The species is typically found in the herb stratum (among grasses and other short plants) and may be collected with a sweep net.

[25] This behavior may include many of the same elements as courtship, such as raising and spreading the first pair of legs and vibrating the abdomen.

[12] Like most spiders, Zygoballus sexpunctatus is an opportunistic feeder, feeding on a wide range of invertebrate prey.

[28] In a study of spider populations in western Tennessee, Zygoballus sexpunctatus spiderlings were reported to hatch from egg sacs in mid summer.

The spiders hibernated through the winter in an immature form and reached sexual maturity around late April.