After a few weeks, Lieutenant Colonel John Murray was appointed to command; he was to remain in this post for most of the regiment's active service.

In 1807, Colonel Isaac Brock, then serving on the staff in North America, reported favourably on the regiment while they were serving as garrison for Quebec City, and commented, "The men were principally raised in the north of Ireland, and are nearly all Protestants; they are robust, active, and good looking.

[3] Major George Taylor captured two 10-gun American vessels, the Growler and Eagle, on 3 June 1813 on the Sorrell River near Île aux Noix on the Canadian side of the lake, after a fight of three-and-a-half hours; British casualties were three men wounded and American casualties were one man killed and eight severely wounded.

Rather than having them all return to England and Ireland, many of soldiers of the 100th Foot were offered and accepted land-grants in Upper Canada.

[8] The transport Hussaren arrived at Quebec from Cowes on 31 May with 124 officers and men of the 76th and 99th Regiments of Foot.