Company I was formed in January 1863 with men from the deactivated Renville Rangers Militia.

Future companies of the regiment such as the Le Seur Tigers fought at New Ulm.

[2] A detachment under Captain Richard Strout first fought at the Battle of Acton and Attack on Hutchinson.

[2] In December Companies A, B, F, G, H, and K were guards at the execution of 38 Santee Sioux in Mankato.

[2] On May 4, 1863, Company G boarded the riverboat Davenport at Fort Snelling as the escort of the first eastern Dakota to leave the Pike island encampment.