It undertook combat operations in Volnovakha Raion where a soldier of the Regiment (Valentyn Viktorovych Drachuk) was allegedly captured near Novotroitske on 30 December 2014 and his dead body was later recovered.

[4] On 1 December 2017, a soldier of the regiment (Oleksandr Yuriyovych Gornyi) was killed in combat in Donetsk Oblast under unknown circumstances.

In total, the regiment destroyed at least 28 Russian targets: eleven fixed wing aircraft, two helicopters, two cruise missiles and thirteen UAVs.

During combat operations near Balaklia and Izium, the "Buk M1" systems destroyed helicopters, two cruise missiles, some planes and UAVs.

[10] During the Russian invasion of Ukraine, the regiment took part in Eastern Ukraine campaign and on 24 March 2022, four personnel (Artem Oleksandrovich Slisarchuk, Yuriy Volodymyrovych Kukuev,[11] Volodymyr Serhiyovych Lutsenko[12] and Bohdan Volodymyrovych Demkiv) of the regiment were killed while performing combat operations in Izium.