The remainder of the regiment was organized in Louisville, Kentucky, and mustered in on September 26, 1862, and mustered in for three years under the command of Colonel Alexander W. Holeman.
The regiment was attached to District of Western Kentucky, Department of the Ohio, to June 1863.
2nd Brigade, 3rd Division, XXIII Corps, Department of the Ohio, to August 1863.
Independent Cavalry Brigade, XXIII Corps, to November 1863.
1st Brigade, 1st Division, Cavalry Corps, Army of the Ohio, to April 1864.
3rd Brigade, Cavalry Division, District of Kentucky, Department of the Ohio, to May 1864.
Independent Brigade, Cavalry Division, XXIII Corps, to September 1864.
Military District of Kentucky, Department of the Ohio, to March 1865.
2nd Brigade, Cavalry Division, District of East Tennessee, Department of the Cumberland, to July 1865.
Duty there and at Bowling Green, Scottsville, and Gallatin, Tennessee, until December 25, 1862.
Expedition to Monticello and operations in southeast Kentucky April 26 – May 12.
Burnside's Campaign in eastern Tennessee August 16 – October 17.
March from Nicholasville, Kentucky, to Dalton, Georgia, April 29 – May 11.
Operations on line Pumpkin Vine Creek and battles about Dallas, New Hope Church, and Allatoona Hills May 25 – June 5.
Operations about Marietta and against Kennesaw Mountain June 10 – July 2.
Ordered to Kentucky August 31, and operating against guerrillas in Green River counties until September.
Burbridge's Expedition into southwest Virginia September 20 – October 17.
Stoneman's Raid into southwest Virginia and western North Carolina March 21 – April 25.