Attached to 3rd Brigade, Abercrombie's Division, Defenses of Washington, to February, 1863.

2nd Brigade, Gordon's Division, Folly Island, S.C., 10th Army Corps, Dept.

Moved to Folly Island, S.C., near Charleston, S.C., harbor August 1–10.

During the fall and winter of 1863 the regiment was engaged at Folly and Morris islands, participating with Gillmore's forces in the siege of Fort Wagner and the bombardment of Fort Sumter and Charleston.

Operations against Charleston and duty on Folly Island, S.C., until December 31.

Expedition to Johns and James Islands against Charleston July 2–10.

Operations against Battery Pringle, Johns Island, July 9.

Moved to Hilton Head, S.C., and duty in that district until June, 1865.

The greatest numbers of casualties incurred by the regiment was during its service on Folly Island during the siege of Charleston, South Carolina.

Contaminated drinking water caused severe illnesses amongst almost the entire regiment.

A convalescent camp was established at St. Augustine, Florida where many of the men spent their illness-caused furloughs.

The most frequent causes of death listed for the many members of the Regiment who died of disease included typhoid fever and chronic diarrhoea.