They were organized at Geneva, Ontario County and mustered in under the command of Colonel William Johnson on September 14, 1862, for a term of service of three years.

[2] In May, 1864, when the unit was in the 2nd (Stedman's) Brigade, 2nd (Weitzel's) Division of the 18th Corps, it took part in the short campaign of the Army of the James under Gen. Butler against Petersburg and Richmond by way of the James River, being engaged at Swift Creek, Proctor's Creek, Drewry's Bluff and Bermuda Hundred.

Corporal E. Van Winkle and privates Henry S. Wells and George A. Buchanan earned the Medal of Honor for their actions at Fort Harrison In October 1864 it was heavily engaged on the old battlefield of Fair Oaks, where it lost 84 killed, wounded and missing.

Corporal E. Van Winkle and Privates Henry S. Wells and George A. Buchanan distinguished themselves at Fort Harrison and were the recipients of the Medal of Honor from the War Department.

[3] The unit mustered out on June 22, 1865, at Richmond, Virginia, under command of Col. Murray and those left with time to serve were transferred to the 100th New York Infantry.