The squadron operates Boeing C-17 Globemaster III aircraft supporting the United States Air Force global reach mission worldwide.

The squadron's aircraft flew supplies to front-line units in Algeria and Tunisia during the North African Campaign as soon as suitable landing strips were available and evacuated casualties back to rear area field hospitals.

During the airborne attack on The Netherlands (Operation Market Garden, September 1944), the squadron dropped paratroops, towed gliders, and flew resupply missions.

When the Allies made the air assault across the Rhine River in March 1945, each aircraft towed two gliders with troops of the 17th Airborne Division and released them near Wesel.

The squadron also hauled food, clothing, medicine, gasoline, ordnance equipment, and other supplies to the front lines and evacuated patients to rear zone hospitals.