They departed Massachusetts on April 4, 1864, arrived at Annapolis and then moved to Camp Marshall in Washington, D.C. On April 22 and 24 they were outfitted with field guns and horses but had virtually no time to train in light artillery tactics before they were assigned to the IX Corps of the Army of the Potomac and joined Lieutenant General Ulysses S. Grant's Overland Campaign.

During the Battle of Totopotomoy Creek on June 1, the 14th Massachusetts Battery played an important role in repulsing a Confederate assault on their section of the lines.

It took an active part in the Siege of Petersburg being moved to the front lines and engaged with enemy artillery from June 21 to 24, July 10 to 17, August 5 to 21 and on October 11.

[6] On April 3, 1865, the day after Confederate troops abandoned Petersburg and retreated westward, the 14th Massachusetts Battery was moved back to City Point.

[6] The battery lost ten men killed in action or mortally wounded nine by disease for a total of 19 dead.