The regiment was attached to Provisional Brigade, Abercrombie's Division, Defenses of Washington, to February 1863.
Foster's Brigade, Division at Suffolk, VII Corps, Department of Virginia, to April 1863.
1st Brigade, Folly Island, Northern District, Department of the South, to February 1864.
2nd Brigade, 3rd Division, X Corps, Army of the James, Department of Virginia and North Carolina, to May 1864.
3rd Brigade, 2nd Division, Terry's Provisional Corps, Department of North Carolina, to March 1865.
3rd Brigade, 2nd Division, X Corps, Army of the Ohio, Department of North Carolina, to July 1865.
The 169th New York Infantry mustered out of service July 19, 1865, at Raleigh, North Carolina.
Expedition from White House to South Anna River July 1–7.
Ordered to the Department of the South, arriving at Folly Island, S.C., July 12.
Operations against Charleston and picket duty on Folly and Black Islands, S.C., until February 1864.
Butler's operations on south side of the James River and against Petersburg and Richmond May 4–28.
Siege operations against Petersburg and Richmond June 16 to December 7.
In the trenches before Petersburg and on the Bermuda Hundred front until August.
Battle of Chaffin's Farm, New Market Heights, September 28–30.