The regiment was attached to 18th Brigade, Army of the Ohio, to March 1862.
District of West Kentucky, Department of the Ohio, to June 1863.
2nd Brigade, 3rd Division, XXIII Corps, Army of the Ohio, to August 1863.
1st Brigade, 3rd Division, XXIII Corps, Department of North Carolina, to July 1865.
The 16th Kentucky Infantry mustered out of service on July 15, 1865.