The 1889–90 Scottish Cup was the 17th season of Scotland's most prestigious football knockout competition.

Queen's Park defeated rivals Vale of Leven 2–1 in a replayed final.

Edinburgh University received a bye to the second round.

Sources:[13][14] Ayr Athletic, Champfleurie, Edinburgh University, Kilsyth Wanderers, Pollokshaws and Wishaw Thistle received a bye to the third round.

Sources:[13][14] Cowdenbeath received a bye to the fourth round.