The 1900 VFL season was the fourth season of the Victorian Football League (VFL), the highest-level senior Australian rules football competition in Victoria.

Fitzroy won its second consecutive minor premiership by finishing atop the home-and-away ladder with an 11–3 win–loss record.

In 1900, the VFL competition consisted of eight teams of 18 on-the-field players each, with no "reserves", although any of the 18 players who had left the playing field for any reason could later resume their place on the field at any time during the match.

If two or more matches in a round are decided by the same margin, these margins are sorted by percentage (i.e. the lowest-scoring winning team is ranked highest and the lowest-scoring losing team is ranked lowest).

Opponents are listed above the margins and home matches are in bold.