All the matches were played in a single leg with eventual replay on the model of the FA Cup; homefields were decided by a draw except for the final match in Florence.

The trophy was won by Genova 1893, which defeated 1–0 Roma in the final match, played at the Giovanni Berta Stadium in Florence on June 6, 1937.

Replay matches * Cerignola and Pistoiese qualify after drawing of lots.

All 16 Serie B clubs are added (Catanzarese, Catania, Hellas Verona, Livorno, Atalanta, Brescia, L'Aquila, Viareggio, Pisa, Messina, Pro Vercelli, Spezia, Cremonese, Palermo, Venezia, Modena).

All 16 Serie A clubs are added (Bari, Milano, Alessandria, Genova 1893, Lazio, Sampierdarenese, Bologna, Fiorentina, Ambrosiana-Inter, Napoli, Lucchese, Juventus, Roma, Triestina, Torino, Novara).